Guides/Peptide Reconstitution Chart
Reference ChartAll Vial Sizes8 Compounds

The Complete Peptide
Reconstitution Chart

Exact BAC water volumes, syringe draw units, and concentrations for every common vial size and dose combination. The definitive reference for reconstituting BPC-157, TB-500, Semaglutide, and all lyophilized peptides.

All values calculated using the universal formula: Concentration (mcg/mL) = Vial (mcg) ÷ BAC water (mL). Draw units for U-100 insulin syringes (100 units/mL).

The Three-Step Reconstitution Formula

Step 1 — Concentration

Vial (mg × 1,000) ÷ BAC water (mL) = mcg/mL

5mg + 2mL = 5,000 ÷ 2 = 2,500 mcg/mL

Step 2 — Draw Volume

Target dose (mcg) ÷ Concentration (mcg/mL) = mL

250mcg ÷ 2,500 = 0.100 mL

Step 3 — Draw Units (U-100)

Draw volume (mL) × 100 = units

0.100 mL × 100 = 10 units

For U-40 syringes (40 units/mL), multiply draw volume by 40 instead of 100. For all other calculations, the formula is identical regardless of peptide compound.

Complete Reconstitution Charts by Vial Size

Find your vial size. Choose your BAC water volume. Read across for the resulting concentration and exact draw units for five common doses. The highlighted row in each table is the most commonly used configuration.

2mg Vial

GHK-Cu, KPV, Sermorelin, Epithalon, CJC-1295

BAC WaterConcentration100mcg200mcg250mcg500mcg1mgDoses/Vial*
0.5mL4,000 mcg/mL2.5 units5 units6.3 units12.5 units25 units8 @ 250mcg
1mL2,000 mcg/mL5 units10 units12.5 units25 units50 units8 @ 250mcg
2mL1,000 mcg/mL10 units20 units25 units50 units100 units8 @ 250mcg
3mL667 mcg/mL15.0 units30.0 units37.5 units75 units150 units8 @ 250mcg

* Doses per vial at 250mcg. U-100 syringe (100 units/mL). Draw values outside 1–100 units shown in grey — adjust BAC water volume for cleaner measurement.

5mg Vial

Most Common

BPC-157, TB-500, Ipamorelin, PT-141, CJC-1295 — most common vial size

BAC WaterConcentration100mcg200mcg250mcg500mcg1mgDoses/Vial*
1mL5,000 mcg/mL2 units4 units5 units10 units20 units20 @ 250mcg
2mL← recommended2,500 mcg/mL4 units8 units10 units20 units40 units20 @ 250mcg
3mL1,667 mcg/mL6 units12 units15 units30 units60 units20 @ 250mcg
4mL1,250 mcg/mL8 units16 units20 units40 units80 units20 @ 250mcg
5mL1,000 mcg/mL10 units20 units25 units50 units100 units20 @ 250mcg

* Doses per vial at 250mcg. U-100 syringe (100 units/mL). Draw values outside 1–100 units shown in grey — adjust BAC water volume for cleaner measurement.

10mg Vial

Epithalon, Tesamorelin, TB-500 loading, PT-141

BAC WaterConcentration100mcg200mcg250mcg500mcg1mgDoses/Vial*
1mL10,000 mcg/mL1 units2 units2.5 units5 units10 units40 @ 250mcg
2mL5,000 mcg/mL2 units4 units5 units10 units20 units40 @ 250mcg
4mL2,500 mcg/mL4 units8 units10 units20 units40 units40 @ 250mcg
5mL2,000 mcg/mL5 units10 units12.5 units25 units50 units40 @ 250mcg
10mL1,000 mcg/mL10 units20 units25 units50 units100 units40 @ 250mcg

* Doses per vial at 250mcg. U-100 syringe (100 units/mL). Draw values outside 1–100 units shown in grey — adjust BAC water volume for cleaner measurement.

20mg Vial

TB-500 loading dose, compound blends, high-dose protocols

BAC WaterConcentration100mcg200mcg250mcg500mcg1mgDoses/Vial*
2mL10,000 mcg/mL1 units2 units2.5 units5 units10 units80 @ 250mcg
4mL5,000 mcg/mL2 units4 units5 units10 units20 units80 @ 250mcg
5mL4,000 mcg/mL2.5 units5 units6.3 units12.5 units25 units80 @ 250mcg
10mL2,000 mcg/mL5 units10 units12.5 units25 units50 units80 @ 250mcg

* Doses per vial at 250mcg. U-100 syringe (100 units/mL). Draw values outside 1–100 units shown in grey — adjust BAC water volume for cleaner measurement.

Compound-Specific Reference

Standard reconstitution setups for the most commonly tracked peptides. Values are reference starting points — your actual BAC water volume should be chosen based on your target dose and preferred draw amount.

BPC-157

5mg vial + 2mL BAC water

10 units
for 250mcg dose
2,500 mcg/mL
Concentration
0.100 mL
Draw Volume
~20
Doses/Vial

Inject SC or IM near injury site. 4–8 week cycles. 20 doses per vial at 250mcg/day.

TB-500

5mg vial + 2mL BAC water

20 units
for 500mcg dose
2,500 mcg/mL
Concentration
0.200 mL
Draw Volume
~10
Doses/Vial

Loading: 5–10mg/week. Maintenance: 2.5–5mg/week. Often stacked with BPC-157.

GHK-Cu

50mg vial + 2mL BAC water

10 units
for 2.5mg dose
25,000 mcg/mL
Concentration
0.100 mL
Draw Volume
~20
Doses/Vial

High-dose vial for GLOW and KLOW blends. 2.5mg doses common.

CJC-1295 No DAC

2mg vial + 2mL BAC water

10 units
for 100mcg dose
1,000 mcg/mL
Concentration
0.100 mL
Draw Volume
~20
Doses/Vial

Short half-life (~30 min). Inject pre-sleep. Stack with Ipamorelin for GH pulse.

Ipamorelin

5mg vial + 2mL BAC water

8 units
for 200mcg dose
2,500 mcg/mL
Concentration
0.080 mL
Draw Volume
~25
Doses/Vial

200–300mcg per injection, 2–3× daily. Minimal cortisol/prolactin elevation.

PT-141

10mg vial + 2mL BAC water

20 units
for 1mg dose
5,000 mcg/mL
Concentration
0.200 mL
Draw Volume
~10
Doses/Vial

1–2mg dose SC 4–6 hours before. Use as needed. 5–10 doses per vial.

Epithalon

10mg vial + 2mL BAC water

2 units
for 100mcg dose
5,000 mcg/mL
Concentration
0.020 mL
Draw Volume
~100
Doses/Vial

100mcg/day for 10-day cycle. 2–3 cycles per year. SC or IV.

Semaglutide

2mg vial + 2mL BAC water

25 units
for 250mcg dose
1,000 mcg/mL
Concentration
0.250 mL
Draw Volume
~8
Doses/Vial

0.25mg starting dose weekly. Titrate per Wegovy schedule. Most compounded sema comes pre-mixed.

How to Reconstitute a Peptide — Step by Step

Complete walkthrough. Approximately 5 minutes per vial.

1

Know your vial size

Your lyophilized peptide vial is labeled in milligrams (mg). The most common sizes are 2mg, 5mg, and 10mg. For all reconstitution math, convert milligrams to micrograms: 1mg = 1,000mcg. A 5mg vial contains 5,000mcg of peptide.

✓ 5mg vial = 5,000mcg total peptide
2

Choose your BAC water volume

The BAC water volume you add determines your concentration — and therefore how many units you'll draw per dose. Use the chart above to find a combination that gives you a clean draw amount. For most protocols with a 5mg vial, 2mL of BAC water is the standard starting point, yielding 2,500 mcg/mL and a 10-unit draw at 250mcg.

✓ 5mg vial + 2mL BAC = 10 units per 250mcg dose
3

Inject BAC water slowly into the vial

Wipe both the peptide vial septum and the BAC water vial septum with a fresh alcohol swab. Draw the correct volume of BAC water into a clean syringe. Insert the needle at a 45° angle through the peptide vial septum. Direct the water stream slowly down the inside wall of the vial — never spray it directly onto the powder. Inject slowly over 10–15 seconds.

✓ Aim for the vial wall, not the powder
4

Swirl gently to dissolve

Once the BAC water is added, gently roll the vial between your palms or swirl in slow circles. Do not shake — vigorous agitation can damage peptide structure. Most peptides dissolve within 30–60 seconds. The solution should be clear and colorless (or very faintly pale). If cloudiness persists, refrigerate and allow additional time to dissolve.

✓ Swirl, never shake. Clear = dissolved.
5

Refrigerate immediately

Reconstituted peptide in bacteriostatic water should be refrigerated at 2–8°C immediately. Do not freeze a reconstituted vial — freeze-thaw cycles degrade peptide structure. Lyophilized (unreconstituted) vials can be stored frozen. Label your reconstituted vial with the reconstitution date. Most peptides in BAC water are stable for 28–30 days refrigerated.

✓ Label with date. Stable 28 days refrigerated.
6

Draw your dose

Find your vial size and BAC water volume in the reconstitution chart above. Read across to your target dose to find your draw units on a U-100 syringe. For 5mg vial + 2mL BAC water at 250mcg dose: draw 10 units. If you have a preferred syringe draw amount, use Custom Draw mode in the free Protocol calculator — enter your units, get the exact BAC water volume.

✓ Use the chart or the free calculator

✦ Custom Draw Mode — Working Backwards

Every reconstitution chart and calculator works in one direction: enter vial size and BAC water, get draw units. But there is a better way.

Custom Draw mode works backwards. Enter your preferred syringe draw amount first — say, 10 units — and the calculator tells you exactly how much BAC water to add to your vial so that 10 units delivers your exact target dose. You choose your preferred draw amount. The math adjusts to match.

Example: 5mg BPC-157, 250mcg target dose, want exactly 10 units:
Draw volume = 10 units ÷ 100 = 0.10 mL
Required concentration = 250mcg ÷ 0.10mL = 2,500 mcg/mL
BAC water to add = 5,000mcg ÷ 2,500 = 2.0 mL ✓
Open Free Peptide Calculator →

Key Reconstitution Facts

Reference facts for peptide reconstitution math. These apply universally to all lyophilized peptide compounds.

1mg = 1,000mcg
All peptide vials are labeled in milligrams. Convert to micrograms (mcg) by multiplying by 1,000. This is the first step in all reconstitution calculations.
U-100 syringe = 100 units per mL
The standard syringe for peptide injections. Each unit equals 0.01mL. A 10-unit draw draws 0.10mL. A 50-unit draw draws 0.50mL.
5mg + 2mL BAC = 2,500 mcg/mL
The most widely used reconstitution setup. 5,000mcg divided by 2mL equals 2,500 mcg/mL concentration. A 250mcg dose requires 10 units on a U-100 syringe.
Stable 28 days refrigerated
Peptides reconstituted with bacteriostatic water are stable for approximately 28–30 days stored at 2–8°C. Always label the vial with the reconstitution date.
BAC water contains 0.9% benzyl alcohol
The benzyl alcohol prevents bacterial growth in multi-dose vials. Do not substitute plain sterile water for multi-dose use — it has no preservative.
Never freeze a reconstituted vial
Freeze-thaw cycles damage peptide structure. Unreconstituted (lyophilized) vials can be stored frozen. Once reconstituted, refrigerate only.
Concentration = mcg ÷ mL (universal formula)
This formula applies to every lyophilized peptide without exception: Concentration (mcg/mL) = Total peptide (mcg) ÷ BAC water added (mL).
Draw units = dose ÷ concentration × 100
The universal draw unit formula for U-100 syringes. Works for any peptide, any vial size, any dose: Draw units = (Target dose mcg ÷ Concentration mcg/mL) × 100.

Frequently Asked Questions

How much BAC water do I add to a 5mg peptide vial?+
The most common amount is 2mL of bacteriostatic water, which produces a concentration of 2,500 mcg/mL. At this concentration, a 250mcg dose requires drawing 10 units on a U-100 insulin syringe. You can use 1mL for a more concentrated solution (5,000 mcg/mL) or 3mL for a more dilute solution (1,667 mcg/mL) — the right amount depends on your target dose and preferred draw volume.
How do I calculate peptide draw units?+
The formula is: Draw units = (Target dose mcg ÷ Concentration mcg/mL) × Syringe units/mL. For a U-100 syringe (100 units/mL): Draw units = Target dose ÷ Concentration × 100. Example: 250mcg dose at 2,500 mcg/mL concentration = (250 ÷ 2500) × 100 = 10 units.
What is bacteriostatic water and why is it used for peptides?+
Bacteriostatic water (BAC water) is sterile water containing 0.9% benzyl alcohol as a preservative. The benzyl alcohol prevents bacterial growth, allowing a reconstituted peptide vial to remain safe for up to 28 days when refrigerated. Regular sterile water has no preservative and should only be used for single-dose administration.
How many units do I draw for a 250mcg dose of BPC-157?+
It depends on how you reconstituted your vial. With a 5mg BPC-157 vial and 2mL of BAC water (concentration 2,500 mcg/mL): draw 10 units on a U-100 syringe. With 1mL BAC water (5,000 mcg/mL): draw 5 units. With 3mL BAC water (1,667 mcg/mL): draw 15 units.
How long does a reconstituted peptide vial last?+
A peptide reconstituted with bacteriostatic water is stable for 28–30 days when stored at 2–8°C (refrigerated). Never freeze a reconstituted vial — freeze-thaw cycles degrade peptide integrity. Store lyophilized (unreconstituted) vials frozen until needed.
What is the concentration formula for peptide reconstitution?+
Concentration (mcg/mL) = Total peptide in vial (mcg) ÷ Volume of BAC water added (mL). To convert mg to mcg, multiply by 1,000. Example: 5mg vial = 5,000mcg. 5,000mcg ÷ 2mL = 2,500 mcg/mL.
Can I use sterile water instead of bacteriostatic water?+
Sterile water can be used for reconstitution but only for single-use vials. Without the benzyl alcohol preservative in BAC water, the reconstituted solution can support bacterial growth and should be used within hours. For multi-dose vials — which most peptide protocols require — bacteriostatic water is the correct diluent.
How much BAC water do I add for semaglutide?+
Compounded semaglutide is typically provided pre-mixed. If reconstituting a lyophilized powder, follow your compounding pharmacy's instructions. For a 2mg semaglutide vial + 2mL BAC water: concentration = 1 mg/mL (1,000 mcg/mL). A 0.25mg starting dose requires drawing 25 units on a U-100 syringe.

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