Research CompoundSubQ · Metabolic

MOTS-c

Mitochondrial Open Reading Frame of the 12S rRNA-c

Half-life
~1-2 hours
Route
SubQ
Typical dose
5–10 mg, 2-3x/week
Reconstitutable
Yes

What is MOTS-c?

MOTS-c is a mitochondrial-derived peptide encoded within the 12S ribosomal RNA of the mitochondrial genome. Discovered in 2015 by researchers at USC, it is one of the first identified mitochondrial-derived peptides with systemic hormonal activity. It acts as a metabolic regulator and exercise mimetic.

MOTS-c activates AMPK (AMP-activated protein kinase) — the cellular energy sensor that drives glucose uptake, fat oxidation, and mitochondrial biogenesis. This AMPK activation produces metabolic effects similar to those of exercise: increased insulin sensitivity, enhanced fat burning, and improved mitochondrial efficiency. Plasma MOTS-c levels decline with age, suggesting a role in metabolic aging.

Research Evidence

SilverInsulin Sensitivity & Glucose Metabolism

Mouse studies demonstrate significant improvements in insulin sensitivity, glucose tolerance, and prevention of diet-induced obesity. AMPK activation is the proposed mechanism.

SilverExercise Mimetic Effects

MOTS-c activates many of the same metabolic pathways as physical exercise at the cellular level. Research suggests it may partially replicate exercise benefits in metabolically compromised subjects.

BronzeLongevity Correlation

Human epidemiological data shows specific MOTS-c variants are associated with longevity in Japanese centenarians. Active research area but causation vs. correlation not yet established.

Evidence grades: Gold = RCT human data · Silver = multiple animal studies, consistent · Bronze = limited or preliminary

Dosing Protocols

Standard dose
5–10 mg SubQ
Community protocols use 5-10mg injected 2-3x per week. Research doses in animal studies are higher on a per-kg basis.
Frequency
2–3x weekly
Monday/Wednesday/Friday or similar. Not daily — the AMPK activation effect has a duration beyond the short half-life.
Timing
Before exercise or morning
Best effects observed when combined with exercise. MOTS-c + exercise appears synergistic.
Stack
GLP-1 protocols
Often stacked with GLP-1 agonists to counteract metabolic slowdown during caloric restriction.

Reconstitution Guide

Vial SizeBAC WaterConcentrationTarget draw
10 mg1 ml10 mg/ml5mg = 5 units
10 mg2 ml5 mg/ml5mg = 10 units
Calculate your exact protocol →

Frequently Asked Questions

What does MOTS-c do?

MOTS-c activates AMPK, the cellular energy sensor, producing effects similar to exercise at the mitochondrial level: improved insulin sensitivity, enhanced fat oxidation, and increased metabolic rate. It is often called an exercise mimetic because it activates many of the same metabolic pathways as physical activity.

Is MOTS-c the same as AOD-9604?

No. Both are used in fat loss protocols but through different mechanisms. AOD-9604 directly activates fat cell receptors for lipolysis. MOTS-c works through AMPK to improve whole-body metabolic efficiency. They are complementary — different mechanisms targeting different aspects of metabolism.

Does MOTS-c work without exercise?

Animal studies show metabolic benefits even without exercise. However, evidence suggests the effects are synergistic with physical activity — MOTS-c + exercise produces greater metabolic improvements than either alone. Exercise is not required but likely amplifies results.

References

  1. [1]Lee C, Zeng J, Drew BG, et al. The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance. Cell Metab. 2015;21(3):443-454.
  2. [2]Reynolds JC, Bhatt DL, Stehlik J, et al. MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis. Nat Commun. 2021;12(1):470.
Disclaimer: This profile is for informational and research purposes only. Not medical advice. Always consult a licensed healthcare provider before using any compound.

This profile was prepared using AI-assisted research synthesis. Citations are provided where applicable — verify with primary sources before clinical application.

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