Research CompoundSubQ · Longevity

Humanin

Humanin — Mitochondrial-Derived Cytoprotective Peptide

Half-life
~2-4 hours
Route
SubQ
Typical dose
2–10 mg, 2-3x/week
Reconstitutable
Yes

What is Humanin?

Humanin is a 21-amino acid mitochondrial-derived peptide encoded within the 16S ribosomal RNA of the mitochondrial genome. Discovered in 2001 by Japanese researchers studying Alzheimer's disease, it is one of the first mitochondrial-derived peptides (MDPs) identified with systemic cytoprotective activity. Humanin levels decline significantly with age and are inversely correlated with age-related disease burden.

Humanin exerts cytoprotective effects through multiple pathways: activation of STAT3 signaling, inhibition of BAX-mediated apoptosis, reduction of amyloid-beta toxicity, improvement of insulin sensitivity, reduction of oxidative stress, and anti-inflammatory signaling. It acts as a systemic survival signal — protecting cells from apoptosis triggered by aging, metabolic stress, and neurotoxic insults. The decline of Humanin with age is hypothesized to contribute to multiple aging pathologies.

Research Evidence

SilverNeuroprotection

Humanin directly inhibits amyloid-beta toxicity to neurons and protects against multiple forms of neuronal apoptosis. Strong evidence for neuroprotective role in Alzheimer's models.

SilverMetabolic Protection

Animal studies demonstrate improved insulin sensitivity, reduced fat accumulation, and protection against metabolic syndrome. Humanin analogs are being developed as metabolic therapeutics.

BronzeLongevity Correlation

Human epidemiological studies show higher circulating Humanin levels correlate with longevity in centenarians' offspring. Causal relationship not yet established by interventional studies.

Evidence grades: Gold = RCT human data · Silver = consistent animal/human data · Bronze = limited or preliminary

Dosing Protocols

Standard dose
2–10 mg SubQ, 2-3x/week
Community protocols use 2-10mg twice weekly. Humanin analogs (HNG) are being studied at lower doses due to higher potency.
Cycle
8–12 weeks, assess response
No established protocol. Short cycles with lab monitoring recommended.

Reconstitution Guide

Vial SizeBAC WaterConcentrationTarget draw
10 mg2 ml5 mg/ml5mg = 10 units
10 mg5 ml2 mg/ml5mg = 25 units
Calculate your exact protocol →

Frequently Asked Questions

How does Humanin compare to MOTS-c?

Both are mitochondrial-derived peptides (MDPs) from the mitochondrial genome. MOTS-c primarily activates AMPK and metabolic pathways (exercise mimetic). Humanin primarily acts as a cytoprotective and neuroprotective signal — inhibiting apoptosis and protecting against neurodegeneration and metabolic stress. They are complementary and are sometimes stacked in comprehensive longevity protocols.

Does Humanin decline with age?

Yes significantly. Circulating Humanin levels decline by approximately 30-40% between young adulthood and old age. This decline correlates with increased susceptibility to apoptotic cell death, metabolic dysfunction, and neurodegeneration that characterize aging. The hypothesis that Humanin supplementation could partially reverse age-related decline is mechanistically plausible but not yet confirmed in human trials.

References

  1. [1]Hashimoto Y, Niikura T, Tajima H, et al. A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Abeta. Proc Natl Acad Sci USA. 2001;98(11):6336-6341.
  2. [2]Lee C, Wan J, Miyazaki B, et al. IGF-I regulates the age-dependent signaling peptide humanin. Aging Cell. 2014;13(5):958-961.
Disclaimer: This profile is for informational and research purposes only. Not medical advice. Always consult a licensed healthcare provider before using any compound.

This profile was prepared using AI-assisted research synthesis. Citations are provided where applicable — verify with primary sources before clinical application.

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